How does serum inactivate trypsin

The optimal proportion for total trypsin inactivation is trypsin 0. No toxic effect was observed for STI at the concentrations used.

Long-term subculturing with this new, alternative dislodging method did not affect cell growth, viability and productivity. Abstract In this work, a BHK21 clone producing a fusion protein consisting of a recombinant human IgG molecule with a cytokine tail, growing in a protein-free medium, was used to test several alternatives to avoid the use of serum for trypsin inactivation, currently used in cell dislodging.

Publication types Research Support, Non-U. When the 5th day fluids were replaced with fresh serum-free medium, cell growth was accelerated and a monolayer was attained by the 7th day. If cells were grown in the absence of whole serum but in the presence of medium containing alpha globulins or fetuin which inhibit both trypsin and cell proteases, such cultures grew as well as cultures containing serum.

The sterilization of trypsin for use in digestion of tissues and cell cultures poses a serious problem. After filtration through 0. A process of purifying and sterilizing trypsin without deleteriously affecting its proteolytic activity is described.